Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic Effects

Over the past decade, cold atmospheric plasma (CAP) has undergone extensive research as a promising therapeutic approach in oncology, with different treatment methods and exposure configurations being investigated resulting various biological effects, most of them after long or durations. This study aimed to evaluate potential custom-made CAP generation source produce plasma-activated medium (PAM) cytotoxic effects subsequently establish optimal parameters. The exposure’s electrical parameters, well pH NO2− content PAM were analyzed. parameters established by evaluating viability human osteosarcoma cells (HOS cell line) osteoblasts (HOB treated under conditions. Our results showed that indirect presents selective dose-dependent while decrease was not found be correlated acidification due exposure. Griess assay revealed very high long-term stable concentrations PAM. Overall, this simple faster method producing on HOS cells, using custom-built source.